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University of Duisburg and Essen, Aquatic Ecosystems Research Group, Universitätsstr. 5, D-45141 Essen, Germany

Monthly Archives: July 2014

Video: DNA extraction

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In this video we show how we homogenise our samples with liquid nitrogen to validate our metabarcoding methods. Once we have grinded the tissues to a fine powder, we divide it up on several reaction tubes and extract the DNA. With DNA barcoding and illumina sequencing we can identify the different species wich were present […]

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2. Metabarcoding analysis

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After successfully completing our first community barcoding analysis, we have started the preparations for a second metabarcoding analysis. Here, we want to test if we can recover the DNA of a variety of different freshwater organisms (resembling a typical macrozoobenthos sample) with our current protocol. This is important to identify bulk macrozoobenthos samples, so molecular data can be […]

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Benthic macroinvertebrate sampling in the Arnsberger Wald

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As a part of the subproject dealing with the species diversity the fauna of several sections of the Kleine Schmalenau, Große Schmalenau and their tributaries has been sampled within the last three weeks. The macroinvertebrates of the more than 20 sampling sites might help to explain the prescence of certain species in both natural and […]

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