The iSeq symposium for Methods and applications of Next Generation Sequencing in evolutionary research took place from the 13th to 14th of November in Leipzig. It was organised by the iDiv centre (http://www.idiv-biodiversity.de). More than hundred scientists from different German universities working with Next Generation Sequencing technics attended this meeting. Two members of the GeneStream Team, Martina Weiss and Hannah Schweyen, as well as two other members from the Department of Animal Ecology, Evolution & Biodiversity, Jan Macher and Andrey Rozenberg, participated in the symposium. They were accompanied by Henning Timm, a member of the Department of Computer Science from the Technische Universität Dortmund. He is currently developing an analysis tool for ddRAD data together with us and Sven Rahman (Genome Informatics, Institute of Human Genetics, University Hospital Essen and University of Duisburg-Essen). The new tool as well as a technic to identify PCR duplicates in ddRAD studies were presented on posters at the meeting.