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University of Duisburg and Essen, Aquatic Ecosystems Research Group, Universitätsstr. 5, D-45141 Essen, Germany

New Lab ‘member’: Tissue homogeniser for metabarcoding samples.

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DNA metabarcoding allows the identification of hundreds of specimens at the same time. This holds great potential for the assessment of freshwater and other ecosystems. However, the appropriate homogenisation of bulk samples for DNA extraction is critical, as we have shown in a recent study. For our future metabarcoding studies using complete benthic kick samples […]

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SWS meeting 2015!

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We also presented our current metabarcoding research on the SWS Annual Meeting 2015 (Providence, Rhode Island, US). Vasco Elbrecht used this opportunity to talk with colleges from the university Guelph and other institutions about current research and future directions in DNA barcoding. We would like to thank the Rub Research School Plus for financing this stay. Video: This video summaries the metabardocing research we […]

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Science Communication course

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Science communication is really important! Vasco Elbrecht has meet up with Eric Francavilla to learn more about science communication. Eric Francavilla works together with scientists at the Pacific Northwest National Laboratory in Richland, Washington and helps scientists to communicate their findings to the public. Like the GeneStream Team, he is also producing YouTube videos. We learned many useful and specific things and […]

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eLearning project receives 5×5000 award!

We are happy to announce that eLearning project “Blended Genome” from Vasco Elbrecht and Florian Leese was awarded with the 5×5000 award of the Ruhr University Bochum. We combine course teaching with video tutorials on YouTube to allow students flexible leaning of handling Next Generation Sequencing data. The first videos are already available on YouTube. Picture: The […]

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Second MiSeq run successful

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The second MiSeq run is now finished. We are testing how many species we can reliably identify with our method. The lab work is now finished and we will analyse the data over the next month. It is planned to get these exiting results published by the end of this year. Additionally we have produced […]

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Video: DNA extraction

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In this video we show how we homogenise our samples with liquid nitrogen to validate our metabarcoding methods. Once we have grinded the tissues to a fine powder, we divide it up on several reaction tubes and extract the DNA. With DNA barcoding and illumina sequencing we can identify the different species wich were present […]

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New video of the mesocosm experiment 2014!

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This year we installed and ran our mesocosm system at the Felderbach (Ruhr area, near Hattingen). In today’s video, we show how we prepared the bottom substrate our experimental channels to represent the habitat of the stream as much as possible. All channels were prepared in the same way. Picture 1: All channels were filled with the […]

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Experiment successfully completed!

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Yesterday, we have successfully completed our mesocosm experiment at the Felderbach in Germany. We started early in the morning to collect all animals, algae and leaf packs from the experimental channels. We and our colleges will analyse and test for a variety of response variables to the different stressors from microbiota to insect communities.Here you […]

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Tag 4 des Aufbaus

Hier ist der Aufbau am 4. Tag im Zeitraffer zu sehen.

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